Differentially expression profile of circular RNAs in hypoxia-preconditioned human placenta chorionic mesenchymal stem cells
SUN Xunsha1 WU Jieying2,3 CHEN Jinsong2,3 LU Yan2,3 YU Qiuxia2,3 LI Ru2,3 WANG Dan2,3 LI Yan2,3 WU Shaoqing2,3▲
1.Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangdong Province, Guangzhou 510080, China;
2.Institute of Perinatology and Birth Health, Guangzhou Women and Children′s Medical Center, Sun Yat-sen University, Guangdong Province, Guangzhou 510623, China;
3.Institute of Perinatology and Birth Health, Guangzhou Women and Children′s Medical Center, Guangzhou Medical University, Guangdong Province, Guangzhou 510623, China
Abstract：Objective To analyze the expression profile variation of circular RNA (circRNA) between hypoxia preconditioned human placenta chorionic mesenchymal stem cells (H-hpcMSCs) and normoxic preconditioned hpcMSCs (N-hpcMSCs). Methods Human circRNA microarray was performed to detect the difference of circRNA expression between 3 paired specimens of H-hpcMSCs and its N-hpcMSCs. The circRNA differental expression and the predicted miRNA of differentially expressed circRNA were analyzed. Results Of the 13 617 circRNAs, 12 114 circRNAs with analytical results. In H-hpcMSCs, a total of 102 circRNAs showed differentially expressed which have more than 1.5 times variation and significant difference (P < 0.05). Among them, 85 increased and change′s fold more than 1.5 times, while 17 reduced and change′s fold more than 1.5 times, a total of 27 circRNAs showed a greater than 2-fold change and all were up-regulated. Five miRNAs of each with differentially expressed circRNA were predicted according to specific base pairing. Conclusion The circRNA expression profile of H-hpcMSCs changed significantly in comparison with N-hpcMSCs, the circRNA may be associated with the hypoxia precondition.
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2018, Vol. 15 
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