Mechanism of Danhong Decoction in protecting cerebral ischemia reperfusion injury
ZHENG Jun1 CHEN Shaoxiu2 ZHANG Xiaochun1
1.Faculty of Pharmaceutical Sciences, Suizhou Central Hospital Suizhou Hospital Affiliated to Hubei Medical College, Hubei Province, Shuizhou 441300, China; 2.Department of Cardiovascular Medicine, Taihe Hospital Taihe Hospital Affiliated to Hubei Medical College, Hubei Province, Shiyan 442000, China
Abstract：Objective To study the protective mechanism of Danhong Decoction on cerebral ischemia-reperfusion injury. Methods The 48 SD rats were divided into MCAO group, positive drug group (Nebivolol) and observation group (Danhong Decoction) by random number table, with 16 cases in each group, which were in accordance with the modified focal cerebral ischemia reperfusion model (MCAO). They were intervened for one week. Before and after intervention, Longa neurological scores were taken to measure cerebral infarct volume and NO, nitric oxide synthase (eNOs), superoxide dismutase (SOD) and malondialdehyde (MDA) in cerebral infarct tissue and Ca2+ content were detected. The endothelin-B (ET-B) receptors in cerebral cortical microglia and the expression of vascular endothelial growth factor (VEGF) protein and its gene in infarcted brain tissue were compared. Results After intervention, the levels of NO, eNOs, SOD, VEGF-mRNA, VEGF protein and Longa neurological score in the observation group were increased, while the levels of MDA, ET-B protein, ET-B mRNA, Ca2+ and infarct volume were decreased, with statistically significant differences (P < 0.05). After intervention, the differences of those indices between the observation group and the MCAO group were statistically significant (P < 0.05). Besides, the differences of VEGF-mRNA, VEGF protein, MDA and SOD levels between the observation group and the positive drug group were statistically significant (P < 0.05). Conclusion Danhong Decoction has a protective effect on cerebral ischemia reperfusion injury.
2018, Vol. 15 
京公网安备 11010502046598号