Abstract：Objective To investigate the curative effect of pricking blood therapy in the treatment of patients with acute gouty arthritis and its influence on serum metabolites. Methods Forty patients with acute gouty arthritis in Outpatient and Inpatient Department of Hangzhou Geriatric Hospital from March 2016 to November 2017 were selected. They were divided into pricking blood group and western medicine group by random number table method, with 20 cases in each group. The pricking blood group was treated with pricking blood therapy, and the western medicine group was given Colchicine Tablets orally, for 1 week respectively. The scores of joint pain index, joint functioning score, blood uric acid and erythrocyte sedimentation rate were evaluated before and after treatment. 10 patients were randomly selected from the pricking blood group. The serum before treatment was taken as group A, and the serum after treatment was taken as group B. Besides 10 healthy people's serum was taken as group D, which were from the volunteers that selected from the healthy employee in the hospital. The serum metabolites of group A, B and D were detected by liquid chromatography - mass spectrometry (LC-MS), and the difference was studied. Results After treatment, serum uric acid, erythrocyte sedimentation rate, joint pain score and joint functioning score of the two groups were significantly lower than those before treatment (P ＜ 0.05), and the joint pain score and joint function score of the pricking blood group were lower than those of the western medicine group (P ＜ 0.05). Tested by metabonomics, the differences in serum metabolites with group A were D- gluconic acid, L-glutamic acid, hypoxanthine, leukotriene B4. And the differences in metabolites between group B and group A were L- arginine, glycerin phospholipid, and insulin like growth factor. Conclusion Pricking blood therapy is better than oral Colchicine in comprehensive efficacy. According to the results of metabonomics detection and KEGG database, we hypothesizes that acute gouty arthritis occurs due to the disturbance of glucose metabolism, amino acid metabolism, hypoxanthine metabolism, and increasing of blood uric acid. And the curative effect of pricking blood therapy is to maintain the patency of the blood vessels and improve the metabolic disorder by affecting the synthesis of nitric oxide and the metabolism of lipids.
2018, Vol. 15 
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