Inhibitory effect of artemisinin on proliferation and invasion of gastric cancer cells
QIAN Bin1 LI Xiaojun1 CHEN Wei1 YIN Xiaochuan2
1.Department of Pharmacy, First Affiliated Hospital of Kunming Medical University, Yunnan Province, Kunming 650032, China;
2.Department of Cerebral Surgery, First Affiliated Hospital of Kunming Medical University, Yunnan Province, Kunming 650032, China
Abstract：Objective To study the effect of artemisinin on the proliferation and invasion of gastric cancer cells. Methods Human gastric cancer cell line SGC-7901 was cultured and dealt with different doses of artemisinin in serum-free DMEM, respectively as 75, 150, 300 μmol/L artemisinin group, serum-free DMEM without artemisinin as control group. After 48 h of intervention, cell proliferation activity, invasion numbers and mRNA expression of proliferation gene, invasion gene were determined. Results After 48 h of intervention, cell proliferation activity, invasion numbers and Ki-67, CyclinD1, CDK4, CDK6, MMP2, MMP9 mRNA expression of 75, 150, 300 μmol/L artemisinin group were significantly lower than those of control group, TIMP1 and TIMP2 mRNA expression were significantly higher than those of control group (P ＜ 0.05), and the higher the dose of artemisinin, the lower the cell proliferation activity, invasion numbers and Ki-67, CyclinD1, CDK4, CDK6, MMP2, MMP9 mRNA expression, the higher the TIMP1 and TIMP2 mRNA expression (P ＜ 0.05). Conclusion Artemisinin can inhibit the proliferation and invasion of gastric cancer cells by inhibiting the expression of cell cycle proteins and matrix metalloproteinases.
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2017, Vol. 14 
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