Abstract：Objective To investigate the effect of 1, 25-dihydroxyvitamin D3 [1, 25 (OH)2D3] on apoptosis of 6T-CEM cell lines and expression of vitamin D receptor (VDR) protein human leukemia. Methods The 6T-CEM cell lines were cultured and randomly divided into group A, B, C, D. Group A, B, C were added with 1, 25 (OH)2D3 with the concentration of 10-6, 10-7, 10-8 mol/L respectively, and group D was added with DMSO. The absorbance A value and cell proliferation inhibition rate were detected by MTT method, the cell morphology was observed by AO-EB fluorescence staining, and the cell apoptosis was detected by flow cytometer, the expression of VDR mRNA and protein were detected by RT-PCR and Western blot method. Results The absorbance of group A was apparently lower than that of group B, C and D (P ＜ 0.05); the inhibitory rate of cell proliferation of group A, B, C was A>B>C; the CEM cell apoptosis index of group D was apparently lower than that of group A, B and C (P ＜ 0.05); the apoptosis index of group A was apparently higher than other three groups (P ＜ 0.05); there was no statistically significant difference in VDR mRNA expression among the four groups (P ＞ 0.05). The VDR protein expression of group A was apparently higher than that of other three groups (P ＜ 0.05). Conclusion 1, 25 (OH)2D3 can effectively inhibit the proliferation of 6T-CEM cells, induce its apoptosis, up-regulate the VDR protein expression.
古丽巴哈·买买提 刘玉 赵莉 严媚. 1，25（OH）2D3对人白血病6T-CEM细胞株凋亡及VDR蛋白表达的影响[J]. 中国医药导报, 2017, 14(18): 12-15.
Gulibaha·Maimaiti LIU Yu ZHAO Li YAN Mei. Effect of 1, 25 (OH)2D3 on apoptosis and VDR protein expression of human leukemia 6T-CEM cell line. 中国医药导报, 2017, 14(18): 12-15.
2017, Vol. 14 
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